Relationship Between Sperm Survival And Oocyte Fertilization


Mahidol University Annual Research Abstracts
Mahidol University Annual Research Abstracts, Vol.

28,
2001
67
RELATIONSHIP BETWEEN SPERM SURVIVAL AND OOCYTE
FERTILIZATION
(NO. 169)
Seesiri Chatasingh, Anna Wongkularb, Somporn Chinsomboon,
Aram Rojanasakul Reproductive Biology Unit, Research
Center and Department of Obstetrics and Gynecology, Faculty of
Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400,
Thailand. Key words : sperm survival, oocyte
fertilization, IVF-ET

Within the last decade, many investigators who worked about
assisted reproductive technology had tried to investigate whether
sperm survival can predict oocyte fertilization. The results were
controversial.

Some proposed that sperm survival was able to
forecast oocyte fertilization to a certain extent . The aim of this
study was to investigate the relationships between rapid,
progressive and motile sperm survival and oocyte fertilization. The
study was performed among 46 couples who came for IVF-ET at
Ramathibodi hospital. Percoll gradient centrifugation technique was
used for sperm preparation.

Sperm parameters were analysed by
computer assisted sperm analyzer (HTM-IVOS Version 10.7, Hamilton
Thorne Research, MA, U.S.A.

). It was found that no relationship was
observed between rapid, progressive and motile sperm survival and
oocyte fertilization . However, when sperm preparation technique in
literature from each group of investigators was reviewed it
appeared that sperm survival from swim up gave some relationship
with oocyte fertilization while that from Percoll gradient
centrifugation did not. Niether did sperm survival in this study.


It was probably concluded that no relationship between rapid,
progressive and motile sperm survival and oocyte fertilization was
revealed. Moreover, different sperm preparation technique, i.e.
swim up and Percoll gradient centrifugation may result in sperm
with different fertilizing capacity.


Iron Intakes and Sources in Healthy Adults
Living in Bangkok and
Sur-rounding
Districts (no. 170) Sriwatana Songchitsomboon1,
Surat komindr2, Noppawan Piaseu2
1 Research Center, 2 Department of
Medicine, Faculty of Medicine, Ramathibodi Hospital, Mahidol
University, Bangkok10400, Thailand Key words : Iron
intake, Dietary assessment, Healthy adults
The levels of intake and food sources of iron in 231
20-80-yr-old healthy adults, living in Bangkok and surrounding
districts were determined. Dietary data from 3-day food records
collected and validated at Research Center, Ramathibodi Hospital
were analyzed. Levels of iron intake were compared between sexes
and age-groups (20-39, 40-59 and 60-80 yr).

Mean daily iron intakes
(+SEM) in men were higher than women (15.3 + 0.4 vs 13.2 + 0.

6
mg/day, p> 0.05), especially mean iron intake in men aged 60-80
yrs were significantly higher than in women of the same age group
(15.6 + 0.8 vs 11.

9 + 0.5 mg/day, p < 0.05). Higher iron intake
from animal sources were found in the youngest (20-39 yr) age-group
of both sexes.

Although, all subjects were recruited as healthy
adults, 20 % of women and 12 % of men were anemia (hemoglobin <
12 mg/dL in female and < 13 mg/dL in male). Significant
correlation were found between hemoglobin and dietary iron intake
(r = 0.149, p < 0.025) and
protein intake (r = 0.

220, p <
0.001). But there were no significant differences in iron intakes
between anemic and non-anemic subjects. The percentage of dietary
iron intake consumed from milk, fish, egg, meat, vegetables,
fruits, legume, condiment and beverage were 2.

3, 6.0, 8.5, 13.3,
10.

9, 29.4, 4.1, 13.5, 16.

1 and 2.6, respectively. Rice and
noodles, the staple food of Thai people accounted for 20.9% of
total dietary iron intake in this population.


Effects of weight loss induced by
dexfenfluramine treatment on obesity-associated health
problems in obese women (No. 171) Supanee
Putadechakum1, Vichai Tanphaichitr2, and
Preeyanuj Yamwong Division of Nutrition and Biochemical
Medicine, Department of Medicine and Research Center, Faculty of
Medicine, Ramathibodi Hospital, Mahidol University, Rama 6 Road,
Bangkok 10400, Thailand. Key words : Dexfenfluramine,
weight
loss, body fat mass, LDL-C, 2-hr postprandial blood
glucose
The effects of weight loss induced by dexfenfluramine treatment
for 12 wks on blood pressure, serum lipid, blood glucose, and serum
uric acid levels were evaluated in 17 obese women, with the mean
(SEM) age of 37.22.

2 yrs, body weight of 83.74.0 kg, body mass
index (BMI) of 34.71.

8 kg/m2, and waist circumference of
95.03.2 cm. Each woman participated in a 28-wk (wks 0-28) study.


Throughout the study, they were advised to reduce their energy
intake with dietary energy distribution of 15-20%
protein-, 20-30% fat-, and 50-65%
carbohydrate-calories. During wks 8-9 and wks 9-20, each woman was
treated daily with 15 and 30 mg of dexfenfluramine,respectively.
Only during the 12-wk dexfenfluramine treatment, there were
persistently significant decreases in their mean body weight, BMI,
body fat mass (BFM), fat-free mass (FFM), and waist circumference;
and their mean net decrease in body weight of 2.9 kg consisting of
65.

5 % of BFM and 34.5 % FFM. After 12 wks of dexfenfluramine
treatment, their mean weight loss of 2.9 kg (3.

5% of body weight at
wk 8) and mean decrease in waist circumference of 2.9 cm (3.1 % of
waist circumference at wk8) lead to significant decreases in serum
total cholesterol (TC), low density lipoprotein-cholesterol
(LDL-C), and 2-hr postprandial blood glucose (PBG) levels. The mean
net decreases in their TC, LDL-C, 2-hr PBG at wk 20 from wk 8 were
13.

3, 8.8, and 20.8 mg/dL, respectively (6.2, 6.

4, and 14.2% of
mean TC, LDL-C, and 2-hr PBG levels at wk 8). Thus even mild weight
loss has significantly improved the aforementioned
obesity-associated health problems in these 17 obese women.
(PISSANULOK 8th world congress of clinical
nutrition 17-20 DEC 2000)

[ Comment, Edit or Article Submission ]



.